BioXcell熱銷產(chǎn)品--BP0273 InVivoPlus anti-mouse PD-1 (CD279)

2023-04-27

瀏覽次數(shù)： 37

BioXcell熱銷產(chǎn)品--BP0273 ?InVivoPlus anti-mouse PD-1 (CD279)

產(chǎn)品描述：

BioXcell InVivoPlus anti-mouse PD-1 29F.1A12單克隆抗體與小鼠PD-1（也稱為CD279）反應(yīng)。PD-1是一種50-55kDa的細(xì)胞表面受體，由Pdcd1基因編碼，屬于Ig超家族的CD28家族。PD-1在CD4和CD8胸腺細(xì)胞以及活化的T和B淋巴細(xì)胞和髓細(xì)胞上瞬時(shí)表達(dá)，在成功消除抗原后PD-1的表達(dá)下降。此外，在B細(xì)胞前階段，Pdcd1 mRNA在發(fā)育中的B淋巴細(xì)胞中表達(dá)。PD-1的結(jié)構(gòu)包括ITIM（基于免疫受體酪氨酸的抑制基序），這表明PD-1負(fù)調(diào)控TCR信號(hào)。PD-1通過結(jié)合B7家族的成員PD-L1和PD-L2發(fā)出信號(hào)。在配體結(jié)合后，PD-1信號(hào)傳導(dǎo)抑制T細(xì)胞活化，導(dǎo)致增殖減少，細(xì)胞因子產(chǎn)生和T細(xì)胞死亡。此外，PD-1敲除動(dòng)物表現(xiàn)出擴(kuò)張型心肌病、脾腫大和外周耐受喪失，在小鼠的外周耐受性和預(yù)防自身免疫性疾病中發(fā)揮關(guān)鍵作用。誘導(dǎo)的PD-L1表達(dá)常見于許多腫瘤，包括鱗狀細(xì)胞癌、結(jié)腸癌和乳腺癌。PD-L1過度表達(dá)會(huì)導(dǎo)致腫瘤細(xì)胞對(duì)CD8T細(xì)胞介導(dǎo)的裂解的抗性增加。在黑素瘤的小鼠模型中，可以通過用阻斷PD-L1與其受體PD-1之間相互作用的抗體治療來暫時(shí)抑制腫瘤生長。由于這些原因，目前正在探索抗PD-1介導(dǎo)的免疫療法作為癌癥治療。與RMP1-14和J43抗體一樣，BioXcell InVivoPlus anti-mouse PD-1 29F.1A12抗體在體內(nèi)可阻斷PD-1與其配體的結(jié)合。

BioXcell熱銷產(chǎn)品--BP0273 InVivoPlus anti-mouse PD-1 (CD279) ?

產(chǎn)品詳情：

產(chǎn)品名稱	InVivoPlus anti-mouse PD-1 (CD279)
產(chǎn)品貨號(hào)	BP0273
產(chǎn)品規(guī)格	5/25/50/100mg
反應(yīng)種屬	Mouse
克隆號(hào)	29F.1A12
同種型	Rat IgG2a
免疫原	Recombinant PD-1-Ig fusion protein
實(shí)驗(yàn)應(yīng)用	in vivo blocking of PD-1/PD-L signaling in vitro PD-1 neutralization Immunohistochemistry (frozen) Immunofluorescence Western blot Flow cytometry
產(chǎn)品形式	PBS, pH 7.0,Contains no stabilizers or preservatives
純度	>95%, Determined by SDS-PAGE
聚合	<5%, Determined by SEC
無菌處理	0.2 μm filtration
純化方式	Protein G
RRID	AB_2687796
分子量	150 kDa
小鼠病原檢測	Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theiler’s Murine Encephalomyelitis: Negative
保存條件	抗體原液保存在4°C，不能冷凍保存。
推薦同型對(duì)照	InVivoPlus rat IgG2a isotype control, anti-trinitrophenol（貨號(hào)BP0089）
推薦抗體稀釋液	InVivoPure pH 7.0 Dilution Buffer（貨號(hào)IP0070）

為什么選擇InVivoPlus抗體？

InVivoPlus級(jí)別的產(chǎn)品內(nèi)毒素含量更低，經(jīng)過多種實(shí)驗(yàn)驗(yàn)證，更適合用于體內(nèi)實(shí)驗(yàn)研究

? BioXcell熱銷產(chǎn)品--BP0273 InVivoPlus anti-mouse PD-1 (CD279)

?該產(chǎn)品自上市已被多篇SCI文獻(xiàn)引用，品質(zhì)有保證，以下是部分已發(fā)表的文獻(xiàn)引用：

應(yīng)用	文章
體內(nèi)PD-1/PD-L信號(hào)阻斷 (in vivo blocking of PD-1/PD-L signaling)	1.?Wang, W., et al. (2018). 'RIP1 Kinase Drives Macrophage-Mediated Adaptive Immune Tolerance in Pancreatic Cancer' Cancer Cell 34(5): 757-774 e757. 2.?Gordon, S. R., et al. (2017). 'PD-1 expression by tumour-associated macrophages inhibits phagocytosis and tumour immunity' Nature 545(7655): 495-499. 3.?Koyama, S., et al. (2016). 'STK11/LKB1 Deficiency Promotes Neutrophil Recruitment and Proinflammatory Cytokine Production to Suppress T-cell Activity in the Lung Tumor Microenvironment' Cancer Res 76(5): 999-1008.
體內(nèi)PD-1/PD-L信號(hào)阻斷，流式細(xì)胞術(shù) (in vivo blocking of PD-1/PD-L signaling, Flow Cytometry)	1.Koyama, S., et al. (2016). 'Adaptive resistance to therapeutic PD-1 blockade is associated with upregulation of alternative immune checkpoints' Nat Commun 7: 10501.
體外PD-1中和（in vitro PD-1 neutralization）	1.Park, S. J., et al. (2014). 'Negative role of inducible PD-1 on survival of activated dendritic cells' J Leukoc Biol 95(4): 621-629.
體內(nèi)PD-1/PD-L信號(hào)阻斷，體外PD-1中和（in vivo blocking of PD-1/PD-L signaling, in vitro PD-1 neutralization）	1.Duraiswamy, J., et al. (2013). 'Dual blockade of PD-1 and CTLA-4 combined with tumor vaccine effectively restores T-cell rejection function in tumors' Cancer Res 73(12): 3591-3603.
流式細(xì)胞術(shù) （Flow Cytometry）	1.Good-Jacobson, K. L., et al. (2012). 'CD80 expression on B cells regulates murine T follicular helper development, germinal center B cell survival, and plasma cell generation' J Immunol 188(9): 4217-4225.