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BioXcell熱銷產(chǎn)品--BE0273 InVivoMab anti-mouse PD-1 (CD279)

2023-04-27
瀏覽次數(shù): 108

BioXcell熱銷產(chǎn)品--BE0273 InVivoMab anti-mouse PD-1 (CD279)

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產(chǎn)品描述:

BioXcell InVivoMab anti-mouse PD-1 29F.1A12單克隆抗體與小鼠PD-1(也稱為CD279)反應(yīng)。PD-1是一種50-55kDa的細(xì)胞表面受體,由Pdcd1基因編碼,屬于Ig超家族的CD28家族。PD-1在CD4和CD8胸腺細(xì)胞以及活化的T和B淋巴細(xì)胞和髓細(xì)胞上瞬時表達(dá),在成功消除抗原后PD-1的表達(dá)下降。此外,在B細(xì)胞前階段,Pdcd1 mRNA在發(fā)育中的B淋巴細(xì)胞中表達(dá)。PD-1的結(jié)構(gòu)包括ITIM(基于免疫受體酪氨酸的抑制基序),這表明PD-1負(fù)調(diào)控TCR信號。PD-1通過結(jié)合B7家族的成員PD-L1和PD-L2發(fā)出信號。在配體結(jié)合后,PD-1信號傳導(dǎo)抑制T細(xì)胞活化,導(dǎo)致增殖減少,細(xì)胞因子產(chǎn)生和T細(xì)胞死亡。此外,PD-1敲除動物表現(xiàn)出擴(kuò)張型心肌病、脾腫大和外周耐受喪失,在小鼠的外周耐受性和預(yù)防自身免疫性疾病中發(fā)揮關(guān)鍵作用。誘導(dǎo)的PD-L1表達(dá)常見于許多腫瘤,包括鱗狀細(xì)胞癌、結(jié)腸癌和乳腺癌。PD-L1過度表達(dá)會導(dǎo)致腫瘤細(xì)胞對CD8T細(xì)胞介導(dǎo)的裂解的抗性增加。在黑素瘤的小鼠模型中,可以通過用阻斷PD-L1與其受體PD-1之間相互作用的抗體治療來暫時抑制腫瘤生長。由于這些原因,目前正在探索抗PD-1介導(dǎo)的免疫療法作為癌癥治療。與RMP1-14和J43抗體一樣,29F.1A12抗體在體內(nèi)阻斷PD-1與其配體的結(jié)合。


BioXcell熱銷產(chǎn)品--BE0273 InVivoMab anti-mouse PD-1 (CD279)?


產(chǎn)品詳情:


產(chǎn)品名稱

InVivoMAb anti-mouse PD-1 (CD279)

產(chǎn)品貨號

BE0273

產(chǎn)品規(guī)格

1/5/25/50/100mg

反應(yīng)種屬

Mouse

克隆號

29F.1A12

同種型

Rat IgG2a

免疫原

Recombinant PD-1-Ig fusion protein

實(shí)驗(yàn)應(yīng)用

in vivo blocking of PD-1/PD-L signaling

in vitro PD-1 neutralization

Immunohistochemistry (frozen)

Immunofluorescence

Western blot

Flow cytometry

產(chǎn)品形式

PBS, pH 7.0,Contains no stabilizers or preservatives

純度

>95%, Determined by SDS-PAGE

無菌處理

0.2 μm filtration

純化方式

Protein G

RRID

AB_2687796

分子量

150 kDa

保存條件

抗體原液保存在4°C,不能冷凍保存。

推薦同型對照

InVivoMAb rat IgG2a isotype control, anti-trinitrophenol(貨號BE0089)

推薦抗體稀釋液

InVivoPure pH 7.0 Dilution Buffer(貨號IP0070)



該產(chǎn)品自上市已被多篇SCI文獻(xiàn)引用,品質(zhì)有保證,以下是部分已發(fā)表的文獻(xiàn)引用:


應(yīng)用

文章

體內(nèi)PD-1/PD-L信號阻斷

(in vivo blocking of PD-1/PD-L signaling)

1.?Wang, W., et al. (2018). 'RIP1 Kinase Drives Macrophage-Mediated Adaptive Immune Tolerance in Pancreatic Cancer' Cancer Cell 34(5): 757-774 e757.

2.?Gordon, S. R., et al. (2017). 'PD-1 expression by tumour-associated macrophages inhibits phagocytosis and tumour immunity' Nature 545(7655): 495-499. ?

3.?Koyama, S., et al. (2016). 'STK11/LKB1 Deficiency Promotes Neutrophil Recruitment and Proinflammatory Cytokine Production to Suppress T-cell Activity in the Lung Tumor Microenvironment' Cancer Res 76(5): 999-1008.

體內(nèi)PD-1/PD-L信號阻斷,流式細(xì)胞術(shù)

(in vivo blocking of PD-1/PD-L signaling, Flow Cytometry)

1.Koyama, S., et al. (2016). 'Adaptive resistance to therapeutic PD-1 blockade is associated with upregulation of alternative immune checkpoints' Nat Commun 7: 10501.

體外PD-1中和

(in vitro PD-1 neutralization)

1.Park, S. J., et al. (2014). 'Negative role of inducible PD-1 on survival of activated dendritic cells' J Leukoc Biol 95(4): 621-629.

體內(nèi)PD-1/PD-L信號阻斷,體外PD-1中和

(in vivo blocking of PD-1/PD-L signaling, in vitro PD-1 neutralization)

1.Duraiswamy, J., et al. (2013). 'Dual blockade of PD-1 and CTLA-4 combined with tumor vaccine effectively restores T-cell rejection function in tumors' Cancer Res 73(12): 3591-3603.

流式細(xì)胞術(shù)

(Flow Cytometry)

1.Good-Jacobson, K. L., et al. (2012). 'CD80 expression on B cells regulates murine T follicular helper development, germinal center B cell survival, and plasma cell generation' J Immunol 188(9): 4217-4225.

免疫熒光,免疫印跡

(Immunofluorescence, Western Blot)

1.Chen, L., et al. (2009). 'Role of the immune modulator programmed cell death-1 during development and apoptosis of mouse retinal ganglion cells' Invest Ophthalmol Vis Sci 50(10): 4941-4948.

免疫組織化學(xué)(冷凍)

(Immunohistochemistry (frozen))

1.?Menke, J., et al. (2007). 'Programmed death 1 ligand (PD-L) 1 and PD-L2 limit autoimmune kidney disease: distinct roles' J Immunol 179(11): 7466-7477.

2.?Liang, S. C., et al. (2003). 'Regulation of PD-1, PD-L1, and PD-L2 expression during normal and autoimmune responses' Eur J Immunol 33(10): 2706-2716.

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BioXcell熱銷產(chǎn)品--BE0273 InVivoMab anti-mouse PD-1 (CD279)

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