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Epicypher熱銷產(chǎn)品——EpiDyne-FRET Nucleosome Remodeling Assay Substrate

2023-10-26
瀏覽次數(shù): 42

當與酶活性重塑復(fù)合物如SMARCA4、SMARCA2和ACF(如EpiCypher貨號15-1014、15-1015、15-1013)配對時,該產(chǎn)品提供核小體重塑的高通量熒光讀數(shù)。EpiDyne?-FRET單核小體由在大腸桿菌中表達的重組人組蛋白(組蛋白H2A-Cy5、H2B、H3.2和H4各兩種;accession numbers::H2A-P4908;H2B-O60814;H3.2-Q71DI3;H4-P62805)組裝而成,該組蛋白由212個DNA堿基對纏繞,并攜帶5'Cy3、Widom 601核小體定位元件和重塑時暴露的DpnII限制性內(nèi)切酶基序(GATC)[1]。H2A在殘基120位處具有Thr-Cys取代,其中Cy5是共軛的。H3.2在殘基110位處具有Cys-to-Ala取代。

[1] Lowary & Widom J Mol. Biol. (1998) PMID: 9514715.


產(chǎn)品詳情

保存溫度:Stable for six (6) months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.

運輸溫度:Dry ice.

產(chǎn)品形式:Purified recombinant mononucleosomes, (21.8 μg protein weight, 50 μg DNA+protein) in 44.2 μL of 10 mM Tris-HCl pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20% glycerol. MW = 240,650 Da. Molarity = 4.7 μM.


驗證數(shù)據(jù)


Epicypher熱銷產(chǎn)品——EpiDyne-FRET Nucleosome Remodeling Assay Substrate

Figure 1: DNA Gel Data

EpiDyne-FRET substrate resolved via native PAGE gel and?

stained with ethidium?bromide to visualize DNA. Lane 1: Free?

DNA (100 ng). Lane 2: Intact EpiDyne-FRET?nucleosomes?

(400 ng).

Epicypher熱銷產(chǎn)品——EpiDyne-FRET Nucleosome Remodeling Assay Substrate

Figure 2: Protein Gel Data

Coomassie stained PAGE gel of proteins in EpiDyne-FRET?

substrate (1 μg)?demonstrates the purity of histones in the?

preparation. Sizes of molecular?weight markers and positions

of the core histones (H2A-Cy5, H2B, H3.2, and H4)?are?

indicated.

Epicypher熱銷產(chǎn)品——EpiDyne-FRET Nucleosome Remodeling Assay Substrate

Figure 3: Nucleosome Remodeling Data

ACF1/ATP-dependent nucleosome remodeling reaction. EpiDyne-FRET?

nucleosomes (15 nM) were?incubated with ACF1 chromatin remodeler

?(EpiCypher 15-1013; indicated concentrations) in the presence?or absence?

of 1 mM ATP. Upon the addition of ATP, reactions were immediately read?

in an Envision Multilabelplate reader (PerkinElmer). Data are presented?

as the mean of the Cy3/Cy5 ratio (N=2).

訂購詳情


貨號

產(chǎn)品名稱

規(guī)格

16-4201? ?

EpiDyne-FRET Nucleosome Remodeling Assay Substrate? ?

50 μg? ?



Epicypher熱銷產(chǎn)品——CUTANA? pAG-Tn5 for CUT&Tag

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