ROS-ID?活性氧/活性氮檢測(cè)試劑盒——ENZO熱銷產(chǎn)品

2023-10-23

瀏覽次數(shù)： 111

自由基和其他活性種在許多心理和病理生理過程中發(fā)揮著重要作用。自由基一旦在細(xì)胞內(nèi)產(chǎn)生，就會(huì)破壞多種細(xì)胞成分，包括蛋白質(zhì)、脂質(zhì)和DNA。然而，在較低濃度下，自由基可以作為細(xì)胞信號(hào)傳導(dǎo)中的第二信使。

Enzo Life Sciences的ROS-ID??ROS/RNS detection kit可用熒光顯微鏡直接實(shí)時(shí)監(jiān)測(cè)活細(xì)胞中活性氧與活性氮（ROS/RNS）的產(chǎn)生。該試劑盒中包含三種檢測(cè)探針：NO檢測(cè)試劑（紅色，Ex/Em 650/670 nm），用于總ROS檢測(cè)的氧化應(yīng)激檢測(cè)試劑（綠色，Ex/Em 490/525 nm）和超氧化物檢測(cè)試劑（橙色，Ex/Em 550/620 nm）。試劑盒內(nèi)還包括陽(yáng)性對(duì)照，綠膿菌素和L-精氨酸，它們分別是ROS和NO產(chǎn)生的常見誘導(dǎo)劑；陰性對(duì)照N-乙酰-L-半胱氨酸和c-PTIO則分別是ROS和NO的常見清除劑。通過將三種檢測(cè)探針與一組特異性抑制劑、激活劑相結(jié)合，能夠區(qū)分超氧化物、一氧化氮和過氧亞硝酸鹽。

產(chǎn)品特點(diǎn)

● 直接監(jiān)測(cè)活細(xì)胞中的活性氧和氮化物（ROS/RNS）

●?可區(qū)分超氧化物、一氧化氮和過氧亞硝酸鹽

●?高靈敏度、特異性和準(zhǔn)確性，可用于活細(xì)胞研究

●?與組織培養(yǎng)基的主要成分（酚紅、FBS和BSA）兼容

●?試劑盒內(nèi)包含整套試劑，包括ROS/RNS誘導(dǎo)劑和清除劑

實(shí)驗(yàn)示例

ROS-ID?活性氧/活性氮檢測(cè)試劑盒——ENZO熱銷產(chǎn)品

圖1. 氧化應(yīng)激檢測(cè)試劑（綠色）、超氧化物檢測(cè)試劑（藍(lán)色）和NO檢測(cè)試劑（紅色）的激發(fā)和發(fā)射光譜。

ROS-ID?活性氧/活性氮檢測(cè)試劑盒——ENZO熱銷產(chǎn)品

圖2. 使用ROS/RNS檢測(cè)試劑盒演示HeLa細(xì)胞中過氧亞硝酸鹽和NO生成的代表性實(shí)驗(yàn)。分析表明，由于NO與超氧化物的反應(yīng)，L-精氨酸和綠膿菌素的聯(lián)合處理產(chǎn)生過氧亞硝酸鹽（綠色熒光），但很少產(chǎn)生NO（紅色熒光）。

ROS-ID?活性氧/活性氮檢測(cè)試劑盒——ENZO熱銷產(chǎn)品

產(chǎn)品信息

產(chǎn)品貨號(hào)	ENZ-51001-200
產(chǎn)品名稱	ROS-ID??ROS/RNS detection kit
別名	Reactive oxygen species / Reactive nitrogen species
規(guī)格	1*1Kit
長(zhǎng)期保存	-80°C
試劑盒組分	NO Detection Reagent (Red), 60μl Oxidative Stress Detection Reagent (Green), 300 nmoles Superoxide Detection Reagent (Orange), 300 nmoles NO Inducer (L-Arginine), 100 μl ROS Inducer (Pyocyanin), 1 μmole NO Scavenger (c-PTIO), 400 nmoles ROS Inhibitor (N-acetyl-L-cysteine), 2x10 mg 10X Wash Buffer, 15 ml
應(yīng)用	Fluorescence microscopy

部分產(chǎn)品引用文獻(xiàn)

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