Epicypher熱銷產(chǎn)品——CTCF CUTANA? CUT&RUN Antibody

2023-09-17

瀏覽次數(shù)： 39

EpiCypher是一家為表觀遺傳學(xué)和染色質(zhì)生物學(xué)研究提供高質(zhì)量試劑和工具的專業(yè)制造商。EpiCypher推出的CUT&RUN級別的CTCF Antibody符合EpiCypher的“CUTANA Compatible”標(biāo)準(zhǔn)，可用于核酸酶靶向切割和釋放（CUT&RUN）和/或靶向剪切及轉(zhuǎn)座酶技術(shù)（CUT&Tag）的基因組定位。CTCF通過結(jié)合DNA并阻止啟動子和附近增強(qiáng)子或沉默子之間的相互作用來調(diào)節(jié)轉(zhuǎn)錄。

產(chǎn)品詳情

反應(yīng)種屬:?Human, Mouse

宿主來源:?Rabbit

實(shí)驗(yàn)應(yīng)用:?CUT&RUN, IHC, ICC, IP, WB

免疫原:?Between amino acids 650 and 700

克隆性:?Monoclonal [BLR041F]

表達(dá)系統(tǒng):?HEK293 cells

保存溫度:?Stable for 1 year at 4°C from date of receipt

運(yùn)輸溫度:?DO NOT FREEZE!! Frozen cold packs.

產(chǎn)品形式:?Purified recombinant monoclonal antibody in borate buffered saline pH 8.2, 0.1% BSA, 0.09% sodium azide

數(shù)據(jù)示例

Epicypher熱銷產(chǎn)品——CTCF CUTANA? CUT&RUN Antibody

Figure 1:?CTCF peaks in CUT&RUN

CUT&RUN was performed as described above. Peaks were called with MACS2. Heatmaps show CTCF peaks relative to IgG negative control antibody in aligned rows ranked by intensity (top to bottom) and colored such that red indicates high localized enrichment and blue denotes background signal. CTCF showed expected enrichment around the TSS. Despite some observable background in the IgG control, differential enrichment with the target antibody is as expected.

Epicypher熱銷產(chǎn)品——CTCF CUTANA? CUT&RUN Antibody ?

Figure 2: CTCF CUT&RUN representative browser tracks

CUT&RUN was performed as described above. Gene browser shots were generated using the Integrative Genomics Viewer (IGV, Broad Institute). Three representative loci are shown.

Epicypher熱銷產(chǎn)品——CTCF CUTANA? CUT&RUN Antibody

Figure 3: Immunohistochemistry data

FFPE sections of human colon carcinoma (left) and mouse spleen (right) using CTCF antibody at a dilution of 1:1000.

Epicypher熱銷產(chǎn)品——CTCF CUTANA? CUT&RUN Antibody

Figure 4: Immunocytochemistry data

FFPE section of human MCF-7 cells examined using CTCF antibody at a dilution of 1:1,000.

Epicypher熱銷產(chǎn)品——CTCF CUTANA? CUT&RUN Antibody

Figure 5: Immunoprecipitation data

EpiCypher CTCF antibody (6?μg/mg lysate) was used to immunoprecipitate whole cell lysates (1 mg, 20% of IP loaded) isolated from HEK293T cells. A negative control IgG antibody and positive control antibodies (Bethyl Laboratories) were also used to demonstrate specificity of the IP. All CTCF antibodies target the same CTCF epitope, however, A300-534A-6 is hosted in rabbit. For blotting immunoprecipitates, EpiCypher CTCF antibody was used at a 1:1,000 dilution.

Epicypher熱銷產(chǎn)品——CTCF CUTANA? CUT&RUN Antibody

Figure 6: Western blot data

Western analysis of CTCF in whole cell extracts from HEK293T, MCF-7, Jurkat, Hep-G2, and A-549 cells. Fifty micrograms of lysate was resolved via SDS-PAGE and detected with a 1:1,000 dilution of CTCF antibody.

訂購詳情