EpiCypher DNA純化試劑盒

2023-04-13

瀏覽次數(shù)： 63

采用專為低洗脫體積（低至6μL）設(shè)計的錐形凈化柱，可回收>50bp的DNA片段，只需30分鐘即可完成純化流程，是各種CUT&RUN靶點，包括組蛋白PTM和轉(zhuǎn)錄因子的理想選擇。?

圖例：

Figure 1: CUT&RUN DNA fragment size distribution analysis

500,000 K562 cells per sample were processed with the CUTANA? ChIC/CUT&RUN Kit (EpiCypher 14-1048) following treatment with IgG negative control (EpiCypher 13-0042), H3K4me3 (EpiCypher 13-0041), H3K27me3 (EpiCypher 13-0030) and CTCF (EpiCypher 13-2014) antibodies. Isolated DNA was used to prepare paired-end Illumina? sequencing libraries, which were analyzed by Agilent TapeStation? , confirming enrichment of mononucleosomes (~300 bp peak represents ~170 bp excised DNA + sequencing adapters).

產(chǎn)品描述：

Cleavage Under Targets & Release Using Nuclease (CUT&RUN) utilizes an immunotethering approach to specifically release genomic fragments of interest into solution, enabling next-generation sequencing and genomic mapping with unprecedented sensitivity. High yield purification and concentration of partitioned chromatin DNA is an essential step - one that requires a workflow specifically compatible with low starting material. Utilizing tapered cleanup columns designed for low elution volumes (as low as 6 μL), this kit produces purified, concentrated DNA ready for library preparation and next-generation sequencing (Figure 1). The user-friendly workflow can be completed in just 30 minutes. The columns recover > 50 bp DNA fragments, making the DNA Purification Kit ideal for a variety of CUT&RUN targets, including histone PTMs and transcription factors.

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