Trilink CleanCap??Reagent AG - (N-7113)產(chǎn)品介紹
mRNA共轉(zhuǎn)錄加帽產(chǎn)品CleanCap??Reagent AG，m7G(5')ppp(5')(2'OMeA)pG
產(chǎn)品描述：
CleanCap??Reagent AG是Trilink專(zhuān)利加帽產(chǎn)品，Trilink N-7113體外共轉(zhuǎn)錄，5’端加帽形成cap1結(jié)構(gòu)。CleanCap的加帽效率可以達(dá)到98%。請(qǐng)注意：Trilink推薦盡可能使用CleanCapAG，CleanCap AG需要AG啟動(dòng)子。通常情況下，CleanCap AG的加帽效率>90%。和CleanCap AG不同，CleanCap GG (cat. no. N-7133 or N-7433) 的專(zhuān)利效率和產(chǎn)量和ARCA類(lèi)似。
產(chǎn)品信息：
產(chǎn)品貨號(hào) | N-7113 |
純度 | ≥95% by AX-HPLC |
消光系數(shù) | 30,539 Lmol-1cm-1?at 255 nm |
分子式 | C32H43N15O24P4?(游離酸) |
分子質(zhì)量 | 1145.60 g/mole (游離酸) |
鹽形式 | Na+ |
濃度 | 100 mM |
溶液組分 | 水 |
推薦的儲(chǔ)存溫度 | -20°C 或者以下 |
其他名稱(chēng) | CleanCap??Reagent AG for co-transcriptional capping of mRNA,m7G(5')ppp(5')(2'OMeA)pG |
應(yīng)用 | CRISPR, In vitro Transcription, Recombinases, TALENS, Transposases, Vaccine Development, Zinc Finger Nucleases |
主鏈 | 5'-5'-三磷酸 |
堿基類(lèi)似物 | 腺苷酸，鳥(niǎo)苷酸 |
核苷酸種類(lèi) | 帽類(lèi)似物 |
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產(chǎn)品規(guī)格如下：
供應(yīng)商 | 產(chǎn)品編號(hào) | 產(chǎn)品名稱(chēng) | 規(guī)格 |
TriLink | N-7113-1 | CleanCap Reagent ?AG, ?1 umole | 1 umol |
TriLink | N-7113-10 | CleanCap Reagent AG, 10 umoles | 10 umol |
TriLink | N-7113-100 | CleanCap Reagent AG, 100 μmole | 100 umol |
TriLink | N-7113-5 | CleanCap Reagent AG, 5 umole | 5 umol |
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文獻(xiàn)引用；
Jiang, T;Henderson, JM;Coote, K;Cheng, Y;Valley, HC;Zhang, XO;Wang, Q;Rhym, LH;Cao, Y;Newby, GA;Bihler, H;Mense, M;Weng, Z;Anderson, DG;McCaffrey, AP;Liu, DR;Xue, W; . Chemical modifications of adenine base editor mRNA and guide RNA expand its application scope
Tusup, M;L . mRNA-Based Anti-TCR CDR3 Tumour Vaccine for T-Cell Lymphoma
Yu, D;Kaur, G;Blumenthal, RM;Zhang, X;Cheng, X; . Enzymatic characterization of three human RNA adenosine methyltransferases reveals diverse substrate affinities and reaction optima
Dobie?y?ska, A;Miszkiewicz, J;Dar?ynkiewicz, ZM;Tyras, M;Stankiewicz-Drogo?, A;Trylska, J;Dar?ynkiewicz, E;Grzela, R; . Development of bis-ANS-based modified fluorescence titration assay for IFIT/RNA studies
Hashimoto, K;Kodama, A;Ohira, M;Kimoto, M;Nakagawa, R;Usui, Y;Ujihara, Y;Hanashima, A;Mohri, S; . Transient induction of cell cycle promoter Fam64a improves cardiac function through regulating Klf15-dependent cardiomyocyte differentiation in mice
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