?產(chǎn)品信息:
經(jīng)Nuclear Fast Red (cat#H-3403)復(fù)染的細(xì)胞核呈粉紅色。其尤其適用于多抗原標(biāo)記的多重染色,可與其他靶標(biāo)蛋白形成鮮明的顏色對(duì)比。此外,該產(chǎn)品為即用型試劑,使用方法簡單,操作快速。
?產(chǎn)品特點(diǎn):
??1. 簡單、快速,一步即可完成染色;
??2. 多抗原標(biāo)記的最佳選擇,可避免蘇木精導(dǎo)致的模糊染色;
??3. 與多種底物相兼容,并形成良好的顏色對(duì)比;
??4. 適用于非水溶性封片劑
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?應(yīng)用舉例:
上圖為腫瘤特異性細(xì)胞質(zhì)的染色結(jié)果(SG,藍(lán)色/灰色),使用Nuclear Fast Red(H-3403)復(fù)染(粉紅色)
?產(chǎn)品訂購信息:
產(chǎn)品 | 貨號(hào) | 規(guī)格 | 性質(zhì) |
Nuclear Fast Red | H-3403 | 500ml | 非水溶性 |
?附 Vector的核復(fù)染試劑與底物的兼容性:
Substrate | Catalog Number | Vector Hematoxylin and Hematoxylin QS H-3401 and H-3404 | Vector Methyl Green H-3402 | Vector Nuclear Fast Red H-3403 |
ImmPACT DAB (brown) | SK-4105 | Excellent Contrast | Excellent Contrast | Fair Contrast |
ImmPACT DAB EqV | SK-4103 | Excellent Contrast | Excellent Contrast | Fair Contrast |
DAB (brown) | SK-4100 | Excellent Contrast | Excellent Contrast | Fair Contrast |
DAB-Ni (gray-black) | SK-4100 | Excellent Contrast | Fair Contrast * | Good Contrast |
ImmPACT AEC (red) | SK-4205 | Excellent Contrast | Counterstain Incompatibility ** | Color Incompatibility |
ImmPACT AMEC Red (red) | SK-4285 | Excellent Contrast | Counterstain Incompatibility ** | Color Incompatibility |
AEC (red) | SK-4200 | Excellent Contrast | Counterstain Incompatibility ** | Color Incompatibility |
TMB (blue) | SK-4400 | Color Incompatibility | Counterstain Incompatibility | Excellent Contrast |
ImmPACT VIP (purple) | SK-4605 | Fair Contrast | Excellent Contrast | Poor Contrast |
Vector VIP (purple) | SK-4600 | Fair Contrast | Excellent Contrast | Poor Contrast |
ImmPACT SG (blue-gray) | SK-4705 | Poor Contrast | Good Contrast | Excellent Contrast |
SG (blue-gray) | SK-4700 | Poor Contrast | Good Contrast | Excellent Contrast |
ImmPACT NovaRED (red) | SK-4805 | Excellent Contrast | Excellent Contrast *** | Color Incompatibility |
Vector NovaRED (red) | SK-4800 | Excellent Contrast | Excellent Contrast *** | Color Incompatibility |
ImmPACT Vector Red (magenta) | SK-5105 | Excellent Contrast | Excellent Contrast | Color Incompatibility |
Vector Red (magenta) | SK-5100 | Excellent Contrast | Excellent Contrast | Color Incompatibility |
Vector Black (black) | SK-5200 | Excellent Contrast | Excellent Contrast * | Excellent Contrast |
Vector Blue (blue) | SK-5300 | Color Incompatibility | Good Contrast | Excellent Contrast |
BCIP/NBT (indigo) | SK-5400 | Color Incompatibility | Excellent Contrast * | Excellent Contrast |
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復(fù)染——作為免疫組化實(shí)驗(yàn)的最后一個(gè)步驟,其重要性不言而喻。
通過復(fù)染,可對(duì)特定的細(xì)胞結(jié)構(gòu)進(jìn)行染色,有助于實(shí)現(xiàn)對(duì)靶標(biāo)蛋白的定位以及可視化觀察,更有利于闡述組織切片形態(tài)學(xué)特征和細(xì)胞內(nèi)部結(jié)構(gòu)。Vectorlabs公司可提供多種核復(fù)染試劑:Hematoxylin、Hematoxylin QS、Methyl Green、Nuclear Fast Red等。產(chǎn)品包裝方便、均為即用型試劑,可用于單個(gè)載玻片或整個(gè)染色皿的染色。
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Vector Laboratories是一家致力于免疫組織化學(xué)、免疫熒光、糖生物學(xué)和生物結(jié)合的標(biāo)記和檢測(cè)試劑研發(fā)和生產(chǎn)的先驅(qū),現(xiàn)已成為該領(lǐng)域的領(lǐng)導(dǎo)者。其產(chǎn)品線包括:ABC免疫組化試劑盒、ImmPRESS多聚合物檢測(cè)試劑盒、防熒光淬滅封片劑、二抗、酶底物、親和素/鏈霉親和素-酶結(jié)合物、封閉試劑等。此外,近期還推出了多抗原染色、新品VectaMount封片劑、M.O.M/H.O.H等特色產(chǎn)品。Vector的設(shè)施通過了ISO?9001:2015認(rèn)證,其研究成果已被350,000多種科學(xué)出版物引用。
?熱銷產(chǎn)品舉例:
產(chǎn)品 | 貨號(hào) | 規(guī)格 | 說明 |
VECTASHIELD? Antifade Mounting Medium with DAPI, 10 ml | H-1200 | 10ml | 最火爆的封片劑 |
ImmEdge? Hydrophobic Barrier (PAP) Pen, 2-Pen set | H-4000 | 2-Pen Set* | 最好用的組化筆 |
VECTASTAIN? Elite ABC-HRP Kit, Peroxidase (Standard), 1 Kit | PK-6100 | 1kit | ABC試劑盒金標(biāo)準(zhǔn) |
DAB Substrate Kit, Peroxidase (HRP), with Nickel, (3,3'-diaminobenzidine), 1 Kit | SK-4100 | 1kit | 性價(jià)比最高的底物 |
Vector? TrueVIEW? Autofluorescence Quenching Kit, 1 kit | SP-8400 | 1kit | 防應(yīng)光淬滅神器 |
Goat Anti-Rabbit IgG Antibody (H+L), Biotinylated, 1.5 mg | BA-1000-1.5 | 1.5mg | 最暢銷的二抗 |
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