產(chǎn)品描述
FLuc mRNA表達(dá)了一個最初從螢火蟲中提取的熒光素酶蛋白。FLuc在底物熒光素存在下發(fā)光，常用于哺乳動物細(xì)胞培養(yǎng)用于測檢測基因表達(dá)和細(xì)胞活力。
FLuc mRNA使用了TriLink特有的共轉(zhuǎn)錄加帽試劑CleanCap進(jìn)行加帽，該加帽試劑可產(chǎn)生Cap 1結(jié)構(gòu)，并具有高效的加帽效率。FLuc mRNA是多聚腺苷酸化的，并針對哺乳動物系統(tǒng)進(jìn)行了優(yōu)化，模仿完全加工的成熟mRNA。
產(chǎn)品信息：
貨號 | L-7602 | 規(guī)格 | 100 μg，1 mg，5 x 1 mg |
純度 | 通過瓊脂糖凝膠遷移實(shí)驗(yàn) | 保存溫度 | -40℃或以下 |
長度 | 1929個核苷酸 | 堿基組成 | 未修飾的堿基 |
換算系數(shù) | 40 μg/OD260 | 溶液 | 1 mM檸檬酸鈉溶液，pH 6.4 |
濃度 | 1.0 mg/mL | 應(yīng)用 | 報(bào)告基因 |
文獻(xiàn)引用：
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Mahiny, Azita Josefine; Karikó, Katalin . Measuring Hematocrit in Mice Injected with In Vitro-Transcribed Erythropoietin mRNA.
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Li, Jiahe; Wang, Wade; He, Yanpu; Li, Yingzhong; Yan, Emily Z.; Zhang, Ketian; Irvine, Darrell J.; Hammond, Paula T. . Structurally Programmed Assembly of Translation Initiation Nanoplex for Superior mRNA Delivery.
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Prieve, Mary G.; Harvie, Pierrot; Monahan, Sean D.; Roy, Debashish; Li, Allen G.; Blevins, Teri L.; Paschal, Amber E.; Waldheim, Matt; Bell, Eric C.; Galperin, Anna; Ella-Menye, Jean-Rene; Houston, Michael E. . Targeted mRNA Therapy for Ornithine Transcarbamylase Deficiency.
Schoellhammer, Carl M.; Lauwers, Gregory Y.; Goettel, Jeremy A.; Oberli, Matthias A.; Cleveland, Cody; Park, June Y.; Minahan, Daniel; Chen, Yiyun; Anderson, Daniel G.; Jaklenec, Ana; Snapper, Scott B.; Langer, Robert; Traverso, Giovanni . Ultrasound-Mediated Delivery of RNA to Colonic Mucosa of Live??? Mice.
Jiang, Yuhang; Gaudin, Alice; Zhang, Junwei; Agarwal, Tushar; Song, Eric; Kauffman, Amy C.; Tietjen, Gregory T.; Wang, Yongheng; Jiang, Zhaozhong; Cheng, Christopher J.; Saltzman, W. Mark . A 'top-down' approach to actuate poly(amine-co-ester) terpolymers for potent and safe mRNA delivery.
Ball, Rebecca L.; Hajj, Khalid A.; Vizelman, Jamie; Bajaj, Palak; Whitehead, Kathryn A. . Lipid Nanoparticle Formulations for Enhanced Co-delivery of siRNA and mRNA.
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