Funakoshi熱銷產(chǎn)品——ThelioKeep

2020-07-23

瀏覽次數(shù)： 52

ThelioKeep是一種保存液，適用于皮膚和神經(jīng)組織保存在4℃。該保存液不含DMSO、血清、蛋白質(zhì)和外來成分。ThelioKeep能保持這些組織的結(jié)構(gòu)強(qiáng)度和活性。?

Funakoshi熱銷產(chǎn)品——ThelioKeep ??

產(chǎn)品特點(diǎn)：?

● ThelioKeep在4℃下保存組織可保存1 - 2周?

●?維持上皮、內(nèi)皮和神經(jīng)組織的形態(tài)和增殖能力。對于外周神經(jīng)，也可以維持電生理功能?

●?ThelioKeep中的茶多酚（(EGCG ; Epigallocatechin gallate）可以保護(hù)薄而脆弱的組織?

●?與多種哺乳動物樣本兼容。?

●?保存在ThelioKeep中的組織可用于各種用途?

●?ThelioKeep中沒有血清，沒有DMSO，沒有蛋白質(zhì)?

●?保證高的細(xì)胞活力，無DMSO細(xì)胞毒性或血清衍生蛋白污染的風(fēng)險(xiǎn)?

●?沒有細(xì)菌、真菌和支原體污染?

●?保質(zhì)期長，ThelioKeep在4℃環(huán)境能穩(wěn)定保存1年時(shí)間?

●?成分是：?Inorganic ions, D-Glucose, Amino Acid, pH adjuster, Epigallocatechin gallate和Phenol Red?

ThelioKeep使用步驟：?

1. ThelioKeep-EGCG的制備:從瓶中取出0.5 ~ 1.0ml?ThelioKeep，在無菌條件下倒入裝有EGCG片劑的管中，將片劑完全溶解在管中。用移液管將全部溶液移回至ThelioKeep瓶中，并充分混合。?

2. 取出適量準(zhǔn)備好的ThelioKeep-EGCG至干凈的試管中，然后冷卻至4~10℃?

3. 將從實(shí)驗(yàn)動物上分離出的上皮或者內(nèi)皮組織浸入到冷卻的ThelioKeep-EGCG中，蓋好蓋子保存在4℃冰箱1~2周?

恢復(fù)步驟：?

1. 在冷的PBS中輕輕清洗保存的組織幾次。將組織轉(zhuǎn)移到實(shí)驗(yàn)介質(zhì)中。?

2. 如果是活體組織用于實(shí)驗(yàn)或培養(yǎng)時(shí)，盡快將其置于生理?xiàng)l件下。?

3. 如果是制備載玻片標(biāo)本時(shí)，從顯微鏡下取出組織后，立即將組織轉(zhuǎn)移到固定劑中。?

4. 當(dāng)從保存的細(xì)胞中提取細(xì)胞質(zhì)物質(zhì)時(shí)，請用冷的PBS充分洗滌細(xì)胞。?

產(chǎn)品使用實(shí)例：?

Funakoshi熱銷產(chǎn)品——ThelioKeep ??

?Human corneal endothelium tissue were preserved for 2 weeks at 4℃.
(1) Left : When using another supplier's tissue culture medium,?
cell morphology was completely disappeared.
(1) Right: When using ThelioKeep, hexagonal shape of the?
cytoplasmic membrane was well maintained.
(2) When ThelioKeepwas used, the cell proliferation speed was?
about twice compared to when another supplier's tissue?
culture medium was used.?

Funakoshi熱銷產(chǎn)品——ThelioKeep

?Rat peripheral nerve tissue preserved for 2 weeks at 4℃were transplanted to another rat and isolated after 24 hours.?
(3) Left : When using another supplier's tissue culture medium,?
structure was completely disrupted.
(3) Right: When using ThelioKeep, structure was well maintained.
(4) When ThelioKeepwas used, electric conductivity was also
maintained as well as fresh tissue.

產(chǎn)品訂購詳情：?