Zeocin是Phleomycin D1的商業(yè)名稱，是從輪枝鏈霉菌Streptomyces CL990分離得到的一種銅螯合的糖肽抗生素，Zeocin通過嵌入DNA使其斷裂而導(dǎo)致細(xì)胞死亡。Zeocin在細(xì)菌、真核微生物、植物細(xì)胞和哺乳動(dòng)物細(xì)胞上均能發(fā)揮活性，用于篩選攜帶Sh ble基因的轉(zhuǎn)染細(xì)胞。
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Zeocin化學(xué)特性
CAS號(hào)：11006-33-0
分子式：C55H85O21N20S2Cu - HCl
分子量：1525
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推薦使用濃度
大腸桿菌
Zeocin抗性的轉(zhuǎn)化子可以在低鹽LB瓊脂糖培養(yǎng)基(yeast extract 5 g/l, Tryptone 10 g/l, NaCl 5 g/l, Agar 15 g/l,pH 7.5)，25 ug/ml?Zeocin的條件下篩選。invivogen公司的FastMedia? Zeo培養(yǎng)基可助您得到最佳篩選效果。
哺乳動(dòng)物細(xì)胞
| 細(xì)胞系 | 種屬 | 組織 | 培養(yǎng)基 | Zeocin(ug/ml) |
| B16 | mouse | melanoma | RPMI | 20-250 |
| CHO | hamster | ovary | DMEM | 100-500 |
| COS | monkey | kidney | DMEM | 100-400 |
| 293 | human | kidney | DMEM | 100-400 |
| Hela | human | uterus | DMEM | 50-100 |
| J558L | mouse | melanoma | RPMI | 400 |
| MCF-7 | human | Breast adenocarcinoma | DMEM | 100-400 |
| MEFs | mouse | embryonic fibroblasts | DMEM | 200-400 |
| THP-1 | human | Leukemic monocytes | RPMI | 200 |
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質(zhì)量控制：
純度>90%(HPLC)
活性經(jīng)微生物學(xué)實(shí)驗(yàn)鑒定
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保存
4°C或-20°C保存1年，避免反復(fù)凍融。
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產(chǎn)品信息:
| 產(chǎn)品名稱 | 產(chǎn)品編號(hào) | 規(guī)格 | 品牌 | 報(bào)價(jià) | 折扣 |
| Zeocin? | ant-zn-1 | 1g (10 x 1 ml) | Invivogen | ￥2,744 | 請(qǐng)?jiān)?/td> |
| Zeocin? | ant-zn-5 | 5g (50 x 1 ml) | Invivogen | ￥10,598 | 請(qǐng)?jiān)?/td> |
| Zeocin? | ant-zn-5b | 5 g (50 ml bottle) | Invivogen | ￥9,884 | 請(qǐng)?jiān)?/td> |
| Zeocin? | ant-zn-1p | 1 g (powder) | Invivogen | ￥2,380 | 請(qǐng)?jiān)?/td> |
| Zeocin? | ant-zn-5p | 5 g (powder) | Invivogen | ￥8,946 | 請(qǐng)?jiān)?/td> |
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文獻(xiàn)引用
?2011 - PNAS, 108: 16663 - 16668
CD147 subunit of lactate/H+ symporters MCT1 and hypoxia-inducible MCT4 is critical for energetics and growth of glycolytic tumors.
?Le Floch R, Chiche J, et al.
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?2011 - Infect. Immun., 79: 1418 - 1427
NOD-like receptor activation by outer membrane vesicles from Vibrio cholerae non-O1 non-O139 strains is modulated by the quorum-sensing regulator HapR.
?Bielig H, Rompikuntal PK, et al.
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?2012 - PNAS, 109: 78 - 83
Combinatorial reshaping of the Candida antarctica lipase A substrate pocket for enantioselectivity using an extremely condensed library.
?Sandstrom AG, Wikmark Y, et al.
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更多基因篩選抗生素信息，請(qǐng)聯(lián)系invivogen中國(guó)戰(zhàn)略合作伙伴——欣博盛生物，全國(guó)服務(wù)熱線：4006-800-892。