Specific Fluorescent probe for Fe2+FeRhoNox?-1?
特異性Fe2+熒光探針?FeRhoNoxTM-1
FeRhoNoxTM-1是特異性檢測Fe2+的熒光探針
該探針能夠特異性地與高爾基體的Fe2+反應(yīng)。能檢測活細(xì)胞高爾基體的Fe2+?
Features?? | 產(chǎn)品特征? |
●Specific?detection?of?Fe2+? | ●?特異性檢測Fe2+? |
●Applicable?to?live?cell?imaging?of?labile?Fe2+? | ●?適用于不穩(wěn)定的Fe2+的活細(xì)胞成像? |
●For?detection?of?Fe2+?in?aqueous?media? | ●?適合于檢測水溶性基質(zhì)里的Fe2+? |
●For?detection?of?labile?iron?in?living?cells? | ●?適合于檢測活細(xì)胞內(nèi)不穩(wěn)定的離子? |
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enceappears aroResponse of FeRhoNox?-1 to Fe2+. Reacting FeRhoNox?-1 with Fe2+for 1 h at 37℃, peak of the fluorescund 575 nm. | Fe2+detection by FeRhoNox?-1in HepG2 cells. HepG2 cells preincubated with/without Fe(NH4)2(SO4)2(FAS: 100 μM) for 0.5 hrwas incubated with 5 μM of FeRhoNox?-1 for 1 hr. |
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The selectivity of FeRhoNox?-1.FeRhoNox?-1 can react with only Fe2+selectively. | Comparison between FeRhoNox?-1’s and Golgi Marker’s staining images. The Merge image shows that FeRhoNox?-1 selectively reacts with Fe2+in Golgi apparatus. |
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Code?no.? | Product?Name? | Target? | Size |
GC?901 | FeRhoNox?-1 | Fe2+in?aqueous?media,?labile?Fe2+in?living?cells | 50μg×10 |
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Application examples using FeRhoNox?-1?
???????????????????????????????????????????? To HepG2 cell?
 ??
|
① - Fe2+ : not added Fe2+?（100 μM Ferrous ammoniumsulfate） | Bpy: cell permeable
Fe2+ selective chelator
(2,20-bipyridyl). |
② +Fe2+ : added Fe2+ |
③ +Fe2+ +Bpy : added Fe2+ and Bpy(1 mM) as Fe2+ chelator |
② shows increasing of FeRhoNox?-1’s fluorescence intensity and |
③showsdecreasing of it. These results indicate that FeRhoNox?-1 reacts with Fe2+ selectively. |
???????????????????????????????????????????? To HEK293 cell
 ?
|
①-Fe2+: not added Fe2+?（100 μMFerrous ammonium sulfate） |
②+Fe2+ ?????????????: added Fe2+ |
③+Fe2+ +Bpy: added Fe2+and Bpy(1 mM) as Fe2+ chelator |
②shows increasing of FeRhoNox?-1’s fluorescence intensity and |
③shows decreasing of it. These results indicate that FeRhoNox?-1?
reacts with Fe2+ selectively. |
???????????????????????????????????????????? Detection?of internal Fe2+
 ?
| Since the fluorescence intensity on?
②appears decreased compared to?
①by adding “Bpy”, FeRhoNox?-1?
detects internal iron(II). |
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