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Expedeon 2019 年新產(chǎn)品 Lightning Link 系列金屬標(biāo)記試劑盒現(xiàn)在有多達(dá)30種啦！

• 手工操作只需30秒！

• 1 小時(shí)內(nèi)完成標(biāo)記

• 無繁瑣的分離步驟，100% 抗體回收率

• 溫和化學(xué)反應(yīng)，標(biāo)記物穩(wěn)定

• 可選金屬多，以便多路測(cè)定

• 批間重復(fù)性好

• 應(yīng)用廣泛

操作步驟

可選標(biāo)記

實(shí)驗(yàn)數(shù)據(jù)

Figure 1. Goat Anti-Rabbit was labeled with Lightning-Link^??159Tb at 100 μg single reaction vial and tested with Rabbit IgG in a sandwich ELISA. Detection: Dissociation-enhanced lanthanide fluorescence.

Figure 2. Goat Anti-Rabbit was labeled with a selection of Lightning-Link^??metal labels at 10 μg single reaction vials. 2 μg conjugates per well were run on a 4-12% Bis-Tris gel (Expedeon, NBT41212), under non-reducing conditions for 20 minutes at 50V and 1 hour at 120V in 1x MOPS (Expedeon, NXB75500). The gel was stained with InstantBlue? (Expedeon, ISB1L) for 1 hour on the gel rocker. The gel showed no free antibody post conjugation for all conjugates except when the reaction was quenched at t=0, where no conjugation was observed.

Figure 3. X-Ray Fluorescence Lα series peaks used to quantify the metal content in Lightning-Link^??Metal Labeling kit.

應(yīng)用

• X-ray fluorescence

• Mass cytometry

• Inductively Coupled Plasma Mass Spectrometry (ICP-MS)

• Dissociation-enhanced fluorescence immunoassays (**DELFIA^?)

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