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Erythrina Cristagalli Lectin (ECL, ECA), Fluorescein, 5 mg/刺桐凝集素，F(xiàn)LUORESCEIN標(biāo)記
品牌： Vectorlabs
產(chǎn)品貨號： FL-1141-5
規(guī)格： 5 mg

報價：
折后價：

數(shù)量： - +
產(chǎn)品詳情
* 以下信息僅供參考，詳情請以原廠網(wǎng)站為準(zhǔn)
  • 產(chǎn)品名稱:
    Erythrina Cristagalli Lectin (ECL, ECA), Fluorescein, 5 mg/刺桐凝集素，F(xiàn)LUORESCEIN標(biāo)記
  • 別名:
    刺桐凝集素，F(xiàn)LUORESCEIN標(biāo)記
  • 產(chǎn)品描述:
    Erythrina cristagalli lectin ECL, ECA consists of two different subunits of approximately 28 kDa and 26 kDa. The carbohydrate structure to which ECL binds is frequently found in membrane and serum glycoproteins of mammalian origin. Sialic acid substitution on this structure appears to prevent the lectin from binding. This specificity offers an opportunity to utilize agarose bound ECL to isolate or fractionate mammalian glycoproteins.This lectin has been reported to be useful for the isolation of human natural killer (NK) cells using a negative selection panning technique (protocol available upon request or on our website). Human NK cells appear to lack accessible surface carbohydrate structures required for binding ECL and, unlike other mononuclear cells, do not adhere to ECL-coated culture dishes. Since this procedure involves a negative selection panning technique, a high recovery of viable NK cells can be obtained. The adherent cells can also be recovered by incubation in galactose or lactose.Fluorescein labeled Erythrina cristagalli lectin has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes. The excitation maximum is at 495 nm and the emission maximum is at 515 nm.Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions perserved with sodium azide.Inhibiting/Eluting Sugar: 200 mM lactose
  • 反應(yīng)種屬:
  • 宿主來源:
  • 實(shí)驗(yàn)應(yīng)用:
    Immunofluorescence,Glycobiology
  • 靶標(biāo)/特異性:
  • 同種型:
  • 推薦稀釋度:
    The recommended concentration range for use is 5-20μg/ml.
  • 免疫原:
  • 免疫原種屬:
  • 克隆性:
  • 克隆號:
  • 純化方式:
  • 偶聯(lián):
    Fluorescein
  • 產(chǎn)品濃度:
    5 mg active conjugate/ml
  • 表達(dá)系統(tǒng):
  • 陽性對照:
  • 保存溫度:
    2° - 8°C – Do Not Freeze
  • 運(yùn)輸溫度:
    Ambient
  • 細(xì)胞定位:
  • 預(yù)期分子量:
  • 產(chǎn)品形式:
  • 存儲溶液:
    10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2
  • 有效期:
  • 產(chǎn)地:
    美國
  • 外觀:
  • 生產(chǎn)商:
  • 功能與背景:
  • URL:
  • 靈敏度:
  • 檢測范圍:
  • 檢測方法:
  • 樣本類型:
    10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2
  • 產(chǎn)品特點(diǎn):
  • 純度:
  • 生物活性:
  • 內(nèi)毒素水平:
  • 來源:
  • 表達(dá)方式:
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  • 分子式:
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  • PubChem CID:
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