BioXCell熱銷(xiāo)產(chǎn)品--CP005 RecombiMAb anti-mouse PD-1 (CD279) (D265A)
產(chǎn)品描述：
29F.1A12?-CP005單克隆抗體是原始29F.1A12?的重組嵌合型抗體?？勺兘Y(jié)構(gòu)域序列與原始29F.1A12?相同，但是恒定區(qū)序列已經(jīng)從大鼠IgG2a變?yōu)樾∈驣gG1。29F.1A12?-CP004抗體在Fc片段中也含有D265A突變，使其無(wú)法與內(nèi)源性Fcγ受體結(jié)合。
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29F.1A12?-CP005單克隆抗體與小鼠PD-1（程序性死亡-1蛋白，也稱(chēng)為CD279）反應(yīng)。PD-1是一種50-55 kDa的細(xì)胞表面受體，由Pdcd1基因編碼，屬于免疫球蛋白超家族的CD28家族。PD-1在CD4和CD8胸腺細(xì)胞以及活化的T和B淋巴細(xì)胞和骨髓細(xì)胞上瞬時(shí)表達(dá)。成功清除抗原后，PD-1表達(dá)下降。此外，Pdcd1 mRNA在前B細(xì)胞階段在發(fā)育中的B淋巴細(xì)胞中表達(dá)。PD-1的結(jié)構(gòu)包括一個(gè)ITIM(免疫受體酪氨酸抑制基序),表明PD-1負(fù)調(diào)節(jié)TCR信號(hào)。PD-1通過(guò)結(jié)合它的兩個(gè)配體PD-L1和PD-L2發(fā)出信號(hào)，這兩個(gè)配體都是B7家族的成員。配體結(jié)合后，PD-1信號(hào)抑制T細(xì)胞活化，導(dǎo)致增殖、細(xì)胞因子產(chǎn)生和T細(xì)胞死亡減少。此外，已知PD-1在小鼠的外周耐受性和預(yù)防自身免疫性疾病中起關(guān)鍵作用，因?yàn)镻D-1敲除動(dòng)物表現(xiàn)出擴(kuò)張性心肌病、脾腫大和外周耐受性喪失。誘導(dǎo)的PD-L1表達(dá)常見(jiàn)于許多腫瘤，包括鱗狀細(xì)胞癌、結(jié)腸腺癌和乳腺腺癌。PD-L1過(guò)度表達(dá)導(dǎo)致腫瘤細(xì)胞對(duì)CD8 T細(xì)胞介導(dǎo)的裂解的抗性增加。在黑色素瘤的小鼠模型中，通過(guò)用阻斷PD-L1和它的受體PD-1之間的相互作用的抗體治療方法，腫瘤生長(zhǎng)可以暫時(shí)被抑制。由于這些研究成果，PD-1目前成為探索抗腫瘤免疫療法的熱門(mén)靶點(diǎn)。
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產(chǎn)品詳情：
產(chǎn)品名稱(chēng) | RecombiMAb anti-mouse PD-1 (CD279) (D265A) |
產(chǎn)品貨號(hào) | CP005 |
產(chǎn)品規(guī)格 | 1mg |
反應(yīng)種屬 | Mouse |
克隆號(hào) | 29F.1A12?-CP005 |
同種型 | Mouse IgG1(switched from rat IgG2a) |
免疫原 | Recombinant PD-1-Ig fusion protein |
實(shí)驗(yàn)應(yīng)用 | in vivo?blocking of PD-1/PD-L signaling*
in vitro?PD-1 neutralization*
Immunohistochemistry (frozen)*
Immunofluorescence*
Western blot*
Flow cytometry*
*Reported for the original rat IgG2a 29F.1A12 antibody |
產(chǎn)品形式 | PBS, pH 7.0,Contains no stabilizers or preservatives |
純度 | >95%, Determined by SDS-PAGE |
聚合 | <5%, Determined by SEC |
無(wú)菌處理 | 0.2 μm filtration |
純化方式 | Protein G |
分子量 | 150 kDa |
小鼠病原檢測(cè) | Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theiler’s Murine Encephalomyelitis: Negative |
保存條件 | 抗體原液保存在4°C，不能冷凍保存。 |
推薦抗體稀釋液 | InVivoPure pH 7.0 Dilution Buffer（貨號(hào)IP0070） |
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該產(chǎn)品自上市已被多篇SCI文獻(xiàn)引用，品質(zhì)有保證，以下是部分已發(fā)表的文獻(xiàn)引用：
應(yīng)用 | 文章 |
體內(nèi)PD-1/PD-L信號(hào)阻斷 (in vivo blocking of? PD-1/PD-L signaling) | 1.?Wang, W., et al. (2018). 'RIP1 Kinase Drives Macrophage-Mediated Adaptive Immune Tolerance in? Pancreatic Cancer' Cancer Cell 34(5): 757-774 e757. 2.?Gordon, S. R., et al. (2017). 'PD-1 expression by tumour-associated macrophages inhibits phagocytosis? and tumour immunity' Nature 545(7655): 495-499. 3.?Koyama, S., et al. (2016). 'STK11/LKB1 Deficiency Promotes Neutrophil Recruitment and Proinflammatory? Cytokine Production to Suppress T-cell Activity in the Lung Tumor Microenvironment' Cancer Res 76(5):? 999-1008. |
體內(nèi)PD-1/PD-L信號(hào)阻斷， 流式細(xì)胞術(shù) (in vivo blocking of? PD-1/PD-L signaling,? Flow Cytometry) | 1.Koyama, S., et al. (2016). 'Adaptive resistance to therapeutic PD-1 blockade is associated with? upregulation of alternative immune checkpoints' Nat Commun 7: 10501. |
體外PD-1中和 （in vitro PD-1 neutralization） | 1.Park, S. J., et al. (2014). 'Negative role of inducible PD-1 on survival of activated dendritic cells' J? Leukoc Biol 95(4): 621-629. |
流式細(xì)胞術(shù) （Flow Cytometry） | 1.Good-Jacobson, K. L., et al. (2012). 'CD80 expression on B cells regulates murine T follicular? helper development, germinal center B cell survival, and plasma cell generation' J Immunol 188(9):? 4217-4225. |
免疫熒光，蛋白質(zhì)印跡 (Immunofluorescence,? Western Blot) | 1.Chen, L., et al. (2009). 'Role of the immune modulator programmed cell death-1 during development and? apoptosis of mouse retinal ganglion cells' Invest Ophthalmol Vis Sci 50(10): 4941-4948. |
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