ENZO公司提供便捷的試劑盒，用于樣本轉(zhuǎn)化和檢測
DNA發(fā)生甲基化會(huì)使基因表達(dá)發(fā)生變化，并影響細(xì)胞的分化，通常會(huì)導(dǎo)致基因組DNA的單向改變。DNA甲基化涉及細(xì)胞的多個(gè)生命進(jìn)程，甲基化若發(fā)生異常會(huì)導(dǎo)致多種疾病的發(fā)生。Enzo拓寬了表觀遺傳分析的產(chǎn)品線，現(xiàn)推出一系列用于DNA甲基化檢測分析的產(chǎn)品。
高效率的DNA 5-mC和5-hmC?轉(zhuǎn)化，確保下游分析的真實(shí)性
· 轉(zhuǎn)化適用于分析單核苷酸序列特異性甲基化情況
· 轉(zhuǎn)化率高，且不造成DNA損傷
· 經(jīng)轉(zhuǎn)化的DNA可通過重亞硫酸鹽測序、甲基化特異性PCR、高分辨率溶解曲線法、芯片檢測及下一代測序檢測
| DNA Only | Blood, Tissue, FFPE, DNA | DNA Only |
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| Express DNA Methylation Kit | Blood & Tissue DNA Methylation Kit | BioArray? 5-hmC Methylation Kit |
| 靶標(biāo) & 修飾 | 5-mC?重亞硫酸鹽修飾 | 5-mC?重亞硫酸鹽修飾 | 5-hmC β-葡萄糖基轉(zhuǎn)移酶protection |
| 形式 | 離心柱 | 96孔板 | 離心柱 | 96孔板 | 離心柱 |
| 洗脫體積 | ≥ 10 μl | ≥ 15 μl | ≥ 10 μl | ≥ 15 μl | ≥ 6 μl |
| 轉(zhuǎn)化效率 | >99.5% | >99.5% | *Enzymatic efficiency ~100% |
| 時(shí)間 | 1.5 h | 4 h | 6-8 h |
| 產(chǎn)量 | 100 pg – 2 μg of DNA | ≥ 50 pg DNA, ≥ 10 cells, blood, tissue, FFPE | 100 ng - 500 ng DNA |
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| 產(chǎn)品名稱 | 產(chǎn)品編號 | 規(guī)格 |
| Express DNA methylation kit?? | ENZ-45001-0050 | 50 tests |
| Express DNA methylation kit(deep well) | ENZ-45002-0002 | 2x96 wells |
| Express DNA methylation kit(shallow well) | ENZ-45001-0002 | 2x96 wells |
| Blood and tissue DNA methylation kit? | ENZ-45004-0050 | 50 tests |
| BioArray? 5-hmC methylation kit? | ENZ-45011-0050 | 50 tests |
| BioArray? methylation DNA IP? | ENZ-45012-0010 | 10 tests |
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DNA甲基化檢測基于實(shí)時(shí)定量PCR的檢測BioPanel??DNA Methylation Detection Kit for Human Pluripotent Stem Cells ·?????????無需重亞硫酸鹽轉(zhuǎn)化
·?????????可識別特定區(qū)域甲基化的百分比，5h內(nèi)完成檢測
·?????????可鑒定在RAB25、NANOG、PTPN6、MGMT、GBP3、LYST基因啟動(dòng)子區(qū)域發(fā)生的DNA甲基化情況
圖1.?人分化型細(xì)胞DNA (黃色柱)，人干細(xì)胞DNA (白色柱)：圖中顯示RAB25、NANOG、PTPN6、MGMT、GBP3及?LYST區(qū)域DNA甲基化的百分比情況。
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| 細(xì)胞類型 | RAB25 | NANOG | PTPN6 | MGMT | GBP3 | LYST |
| 分化型細(xì)胞 | + | + | + | - | - | - |
| 多能干細(xì)胞 | - | - | - | + | + | + |
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基于ELISA的檢測5-Methylcytosine DNA ELISA kit ·?????????3h內(nèi)即可完成對任何DNA樣本內(nèi)5-mC的精確定量
·?????????高通量分析檢測的理想工具
·?????????特異性高，與LC-MS/MS-MRM分析結(jié)果一致
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圖2.?基因組DNA分析：質(zhì)譜法(MS)??VS??ELISA法.采用5-mC DNA ELISA Kit (prod. no. ADI-900-224)?定量檢測多個(gè)DNA樣本中5-mC含量，與LC-MS/MS-MRM法檢測結(jié)果高度相關(guān)?；蚪MDNA?樣本包括：人大腦組織(HB)，人腎組織?(HK),?人胚胎干細(xì)胞(HESC)，小鼠大腦組織?(MB)，小鼠腎組織(MK)，小鼠睪丸?(MT)。
| 產(chǎn)品名稱 | 產(chǎn)品編號 | 規(guī)格 |
| BioPanel? methylation detection kit for human pluripotent stem cells (Light Cycler? 480 RT PCR) | ENZ-45007-0001 | 96 wells |
| BioPanel? methylation detection kit for human pluripotent stem cells (CFX Touch? RT PCR) | ENZ-45008-0001 | 96 wells |
| BioPanel? methylation detection kit for human pluripotent stem cells (7500? or 7900? RT PCR) | ENZ-45009-0001 | 96 wells |
| BioPanel? methylation detection kit for human pluripotent stem cells (custom format)? | ENZ-45010-0048 | 48 tests |
| 5-Methylcytosine DNA ELISA kit? | ADI-900-224-0001 | 96 wells |
| 5-Hydroxymethylcytosine DNA ELISA kit | ADI-900-225-0001 | 96 wells |
| Universal methylated DNA standard (human)? | ENZ-45005-0001 | 20 tests |
| Universal methylated DNA standard (mouse) | ENZ-45006-0001 | 20 tests |
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詳情請咨詢ENZO中國授權(quán)代理商——欣博盛生物科技有限公司。?