Epicypher新品 | 核酸提取緩沖液

2023-11-22

瀏覽次數(shù)： 118

Epicypher新品 | 核酸提取緩沖液

CUTANA? 細(xì)胞核提取緩沖液是CUT&RUN和CUT&Tag實(shí)驗(yàn)中，用于從培養(yǎng)的細(xì)胞和組織中提取細(xì)胞核的必需試劑。這種緩沖液可與多種真核細(xì)胞和組織兼容。以下是使用該緩沖液成功進(jìn)行細(xì)胞核分離的細(xì)胞和組織類(lèi)型：

mouse NIH3T3 fibroblast cells	human A549 non-small cell lung cancer (NSCLC) cells
human K562 leukemia cells	human NCI-H1299 non-small cell lung cancer (NSCLS cells)
human bone marrow derived macrophages	human TIG-1 fetal lung cells
human monocyte derived macrophages	human LoVo colorectal cancer cells
human MV-4-11 macrophage cells	human LNCaP prostate carcinoma cells
human SUM149 triple negative breast cancer (TNBC) cells	human renal primary cells
human GM24385 B-lymphocyte (aka HG002) cells	human peripheral blood mononuclear cells (PBMCs)
human MCF7 breast cancer cells	human intestinal tissue
human MDA-MB-231 breast cancer cells	human HEPM embryonic cells
human SK-MEL-2 melanoma cells

只需將試劑盒中的Pre-Nuclei Extraction Buffer和亞精胺與蛋白酶抑制劑混合即可完成該試劑的制備。CUTANA? 細(xì)胞核提取緩沖液針對(duì)CUT&RUN和CUT&Tag實(shí)驗(yàn)流程進(jìn)行了優(yōu)化，從而獲得高質(zhì)量的細(xì)胞核，助力您的基因組圖譜分析。

試劑盒組成

組分	貨號(hào)
CUTANA??Pre-Nuclei Extraction Buffer? ? ??	21-1026a? ? ?
1 M Spermidine	21-1026b

保存條件

將Pre-Nuclei Extraction Buffer 4oC儲(chǔ)存, 1 M亞精胺-20oC儲(chǔ)存。

自收到之日起可穩(wěn)定儲(chǔ)存6個(gè)月。

驗(yàn)證數(shù)據(jù)

Epicypher新品 | 核酸提取緩沖液

Figure 1: Nuclei Extraction

Nuclei were extracted from 3 different cell types: K562, NIH3T3, and LNCaP using the CUTANA? Nuclei Extraction Protocol for CUT&RUN and CUT&Tag. Top 3 panels show cells before extraction. Starting cells are viable (bright white and round). Bottom 3 panels show nuclei extracted using the CUTANA? Nuclei Extraction Buffer as indicated by positive Trypan Blue staining.

訂購(gòu)詳情