CUTANATM?pAG-MNase是進行染色質(zhì)免疫切割(ChIC)和核酸酶靶向切割和釋放(CUT&RUN)的關(guān)鍵試劑。作為蛋白A/G與微球菌核酸酶的融合表達產(chǎn)物，CUTANA pAGMNase與來自各種物種宿主的靶抗體兼容，并且經(jīng)過高度純化以去除污染的E. coli?DNA，這可能會使細胞數(shù)量少的樣本分析復(fù)雜化。與ChIP-seq相比，pAG-MNase可以在ChIC/CUT&RUN中有效地繪制染色質(zhì)特征，可以顯著改善信噪比和測序深度。
保存條件
Stable for one year at -20°C from date of receipt.
數(shù)據(jù)示例
|
FIGURE 1： CUT&RUN gene browser tracks.?CUT&RUN was performed as described above. Data verifies low non-specific MNase digestion with the absence of peaks in the IgG track, an expected H3K4me3 profile with sharp promoter peaks, and broad peaks in heterochromatin regions consistent with H3K27me3. Image was generated using the Integrative Genomics Viewer (IGV, Broad Institute). |
 ?
|
FIGURE 2： Size distribution of released chromatin. CUT&RUN was performed as described above. Excised DNA is highly enriched for mononucleosomes (peaks at ~300 bp represent 150 bp nucleosomes + sequencing adapters). |
 ?
|
FIGURE 3： CUT&RUN genome-wide heatmaps. CUT&RUN was performed as described above. Heatmaps show CUT&RUN signal aligned to annotated transcription start sites (TSS, +/- 2kb). High and low signal are ranked by intensity (top to bottom) and colored such that red indicates high localized enrichment and blue denotes background signal. Gene rows in each heatmap are aligned and sorted from high to low signal relative to H3K4me3 (middle). |
|
FIGURE 4： Protein gel data. CUTANATM pAG-MNase (1 μg) was resolved via SDS-PAGE and stained with Coomassie blue. The migration and molecular weight of the protein standards are indicated. |
訂購詳情
貨號 | 產(chǎn)品名稱 | 規(guī)格 |
15-1016 | CUTANA? pAG-MNase for ChIC/CUT&RUN Workflows | 50 Reactions |
15-1116 | CUTANA? pAG-MNase for ChIC/CUT&RUN Workflows | 250 Reactions |
?
如需了解更多詳細信息或相關(guān)產(chǎn)品，請聯(lián)系EpiCypher中國授權(quán)代理商-欣博盛生物?
全國服務(wù)熱線: 4006-800-892 ? ? ??郵箱: market@neobioscience.com?
深圳: 0755-26755892 ? ? ? ??北京: 010-88594029 ???????????
廣州：020-87615159????????? ?上海: 021-34613729
代理品牌網(wǎng)站: m.smblzp.com?
自主品牌網(wǎng)站: www.neobioscience.net