GFP-CERTIFIED?FLUOFORTE?鈣離子檢測試劑盒——ENZO熱銷產(chǎn)品

2023-10-23

瀏覽次數(shù)： 37

Enzo Life Sciences的GFP-CERTIFIED^??FLUOFORTE^??Calcium assay kit提供了一種熒光分析方法，用于檢測廣泛生物靶標(biāo)的細(xì)胞內(nèi)鈣動(dòng)員情況。相對(duì)于其他商業(yè)化的染料，GFP-CERTIFIED^??FLUOFORTE^?染料是最亮和最靈敏的熒光鈣指示劑。該試劑盒為貼壁和非貼壁細(xì)胞系提供了更少步驟，更低變異性，更簡易的鈣檢測。此外，它既不需要洗滌步驟，也不需要添加外源性淬滅染料，避免了對(duì)受體-配體相互作用動(dòng)力學(xué)的干擾。

Enzo Life Sciences的GFP-CERTIFIED^??FLUOFORTE^??Calcium assay kit可用于細(xì)胞內(nèi)鈣離子的高通量檢測，鈣離子是重要的第二信使，常用于初步篩選中監(jiān)測G蛋白偶聯(lián)受體激活。

產(chǎn)品特色

● 可監(jiān)測表達(dá)GFP細(xì)胞中的鈣離子動(dòng)員情況

●?最明亮、最靈敏的熒光Ca2+指示劑

●?獲得的 EC50和IC50值與使用鈣離子熒光探針 Rhod-4 AM和Fluo-4 AM 相當(dāng)

●?更大范圍的檢測窗口

●?使用方便，無需洗滌，無需淬滅染料

實(shí)驗(yàn)示例

GFP-CERTIFIED?FLUOFORTE?鈣離子檢測試劑盒——ENZO熱銷產(chǎn)品

GFP-CERTIFIED?FLUOFORTE?鈣離子檢測試劑盒——ENZO熱銷產(chǎn)品 ?

圖2. U-2 OS細(xì)胞分別與（A）GFP-CERTIFIED^??FLUOFORTE?AM染料和（B）Rhod-2 AM染料孵育。洗滌細(xì)胞并用終濃度為100nM的ATP處理，并立即成像。GFP-CERTIFIED^??FLUOFORTE^??AM染料比Rhod-2 AM表現(xiàn)出更亮的熒光強(qiáng)度。

產(chǎn)品信息

產(chǎn)品貨號(hào)	ENZ-51020-KP010/ ENZ-51020-KP100
產(chǎn)品名稱	GFP-CERTIFIED^??FLUOFORTE^??Calcium assay kit
規(guī)? ? ? ?格	1096wells/10096wells
短期保存	-20°C
長期保存	-80°C
試劑盒組分	Starter pack (Prod. No. ENZ-51020-KP010): Reagent A (lyophilized GFP-CERTIFIED^??FLUOFORTE^??dye), 1 vial Reagent B (dye efflux inhibitor), 10ml Reagent C (Hanks’ buffer with 20mM HEPES, 100ml) Sufficient for 10 x 96-well plates High-throughput (Prod. No. ENZ-51020-KP100): Reagent A (lyophilized GFP-CERTIFIED^??FLUOFORTE^??dye), 10 vials Reagent B (dye efflux inhibitor), 10 x 10ml Sufficient for 100 x 96-well plates

部分產(chǎn)品引用文獻(xiàn)

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10.?Defects in T-tubular electrical activity underlie local alterations of calcium release in heart failure: C. Crocini, et al.; PNAS 111, 15196 (2014)

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