ENZO熱銷產(chǎn)品——VEGF (human), ELISA kit

2023-05-30

瀏覽次數(shù)： 54

血管內(nèi)皮生長因子(VEGF)最初被稱為血管通透性因子(VPF)，是一個刺激血管發(fā)生和血管新生的蛋白質(zhì)家族。VEGF家族包括五個成員：VEGF-A、VEGF-B、VEGF-C、VEGF-D和PGF（胎盤生長因子）。作為第一個被發(fā)現(xiàn)的VEGF家族成員，VEGF-A常被簡稱為VEGF。VEGF-A基因由8個外顯子組成，選擇性剪接產(chǎn)生10種以上亞型，包括VEGF 121、VEGF 145、VEGF 165、VEGF 183、VEGF 189和VEGF 206等。VEGF165和VEGF121是在大多數(shù)細(xì)胞和組織中表達(dá)的主要亞型。VEGF165是一種45KDa同源二聚體糖蛋白，對肝素具有中等親和力。相反，VEGF121不與肝素結(jié)合。VEGF特異性結(jié)合酪氨酸激酶受體VEGFR-1(Flt-1)和VEGFR-2(Flk-1)，激活下游信號通路，導(dǎo)致內(nèi)皮細(xì)胞存活、增殖、遷移和血管通透性。VEGF通過增強血管通透性或招募內(nèi)皮祖細(xì)胞到新生血管形成部位遠(yuǎn)處位點，在病理性血管生成中起著核心作用。隨著對VEGF促進病理性血管生成和腫瘤轉(zhuǎn)移的深入了解，在多種臨床模型中，阻斷VEGF/VEGFR軸抑制血管生成和腫瘤生長已成為合理的研究方向。

ENZO VEGF ELISA kit是一種靈敏的比色酶免疫測定試劑盒，可在2.5小時內(nèi)得到檢測結(jié)果。在450 nm處讀取吸光度。

●?高靈敏度，可檢測低至4.712 pg/ml的VEGF

●?高特異性，與VEGF 165的交叉反應(yīng)為100%，與包括VEGF 121在內(nèi)的相關(guān)分子幾乎沒有交叉反應(yīng)

●?高通量，可檢測多達(dá)38個樣本（帶復(fù)孔），僅需2.5h即可獲得結(jié)果

●?彩色編碼試劑有助于減少錯誤并提高效率

ENZO熱銷產(chǎn)品——VEGF (human), ELISA kit

產(chǎn)品信息

產(chǎn)品貨號	ENZ-KIT156-0001
產(chǎn)品名稱	VEGF (human), ELISA kit（人血管內(nèi)皮生長因子ELISA試劑盒）
規(guī)格	96 wells
其他名稱	Vascular endothelial growth factor
靈敏度	4.712 pg/ml
檢測范圍	8-2,000 pg/ml
檢測時間	2.5 hours
樣本種屬	Human
樣本類型	human serum, plasma, and culture supernatants
保存溫度	+4°C
試劑盒組分	Microtiter Plate, Conjugate, Detector Antibody, Assay buffer, Antibody Diluent, Wash Buffer Concentrate, Standard, TMB Substrate, Stop Solution

部分產(chǎn)品文獻(xiàn)引用

1. Negative Modulation of the Angiogenic Cascade Induced by Allosteric Kinesin Eg5 Inhibitors in a Gastric Adenocarcinoma In Vitro Model: A. Ricci, et al.; Molecules 27, 957 (2022)?

2. Attenuation of high glucose-induced damage in RPE cells through p38 MAPK signaling pathway inhibition: G. Maugeri, et al.; Front. Pharmacol. 12, 684680 (2021), Application(s): ELISA using culture supernatants?

3. Mifepristone as a potential therapy to reduce angiogenesis and p-glycoprotein associated with glioblastoma resistance to temozolomide: M. Llaguno-Munive, et al.; Front. Oncol. 10, 581814 (2020)?

4. The combination of sulforaphane and Fernblock? XP improves individual beneficial effects in normal and neoplastic human skin cell lines: S. Serini, et al.; Nutrients 12, 1608 (2020), Application(s): ELISA using culture supernatants

5. Aflibercept regulates retinal inflammation elicited by high glucose via the PlGF/ERK pathway: F. Lazzara, et al.; Biochem. Pharmacol. 168, 341 (2019), Application(s): ELISA using culture supernatants

6. Mechanism of the anti-angiogenic effect of avemar on tumor cells: N.G. Imir, et al.; Oncol. Lett. 15, 2673 (2018), Application(s): ELISA using culture supernatants?

7. The Role of Human Umbilical Vein Endothelial Cells in Osteogenic Differentiation of Dental Follicle-Derived Stem Cells in In Vitro Co-cultures: J.S. Bok, et al.; Int. J. Med. Sci. 15, 1160 (2018)

8. The effect of etanercept on vascular endothelial growth factor production by cutaneous mesenchymal stem cells from patients with psoriasis: A. Campanati, et al.; J. Int. Med. Res. 44, 6 (2016), Application(s): VEGF quantification, human MSC cells?

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