報告蛋白通過酶催化或熒光直讀的方式來監(jiān)測細胞轉(zhuǎn)染、蛋白表達模式以及啟動子功能。這些報告蛋白可以直接檢測,或者像下圖利用報告蛋白特異性抗體對報告蛋白進行western blot檢測。
使用mouse anti-GST(MCA1352)和anti-mouse IgG-HRP(STAR13B )對GST-ubiquitin 進行Western blot檢測
AbD Serotec公司最受歡迎的的報告蛋白抗體
?產(chǎn)品名稱 | ?克隆號 | ?形式 | ?應(yīng)用 | ?產(chǎn)品編號 |
?B- ?Galactosidase | ?4722 HuCAL | ?Purified | ?E | ?HCA033 |
?4723 HuCAL | ?Purified | ?E | ?HCA034 |
?4724 HuCAL | ?Purified | E | ?HCA035 |
?Green ?Fluorescent ?Protein (GFP) | ?264-449-2 | ?Purified | ?C, IB, IP | ?OBT1643 |
?338 HuCAL | ?Purified | ?E | ?HCA060 |
?N/K | ?Purified | ?C, E, P, WB | ?OBT1820 |
?Poly | ?Purified | ?E, IF | ?4745-1051 |
?Poly | ?Purified | ?E, IF, WB | ?AHP975 |
?Poly | ?Purified | ?E, IB | ?OBT1645 |
?Glutathione-S- ?Transferase ?(GST) | ?2021 HuCAL | ?Purified | ?E, WB | ?HCA055 |
?2031 HuCAL | ?Purified, FITC, HRP | ?E, WB | ?HCA056 |
?3937 HuCAL | ?Purified | ?E, WB | ?HCA054 |
?Vpg66 | ?S/N,Biotin | ?E, IF, WB | ?MCA1352 |
?Luciferase | ?BGN/06/942 ? | ?Purified | ?E | ?5709-4360 |
?BGN/06/944 | ?Purified | ?E | ?5709-4380 |
?LUC-Y | ?Purified | ?C, E, F, WB | ?MCA2076 |