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BioXcell熱銷產(chǎn)品--RecombiMAb anti-mouse PD-1 (CD279) (D265A)

2023-10-15
瀏覽次數(shù): 37


BioXcell熱銷產(chǎn)品--RecombiMAb anti-mouse PD-1 (CD279) (D265A)


產(chǎn)品描述:

RMP1-14-CP002單克隆抗體是原始RMP1-14克隆號的重組嵌合型抗體。可變結(jié)構(gòu)域序列與原始RMP1-14克隆號相同,但是恒定區(qū)序列已經(jīng)從大鼠IgG2a變?yōu)樾∈驣gG1。RMP1-14-CP002單克隆抗體在Fc片段中也含有D265A突變,使其無法與內(nèi)源性Fcγ受體結(jié)合。

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RMP1-14-CP002單克隆抗體與小鼠PD-1(程序性死亡-1蛋白,也稱為CD279)反應(yīng)。PD-1是一種50-55 kDa的細(xì)胞表面受體,由Pdcd1基因編碼,屬于免疫球蛋白超家族的CD28家族。PD-1在CD4和CD8胸腺細(xì)胞以及活化的T和B淋巴細(xì)胞和骨髓細(xì)胞上瞬時表達(dá)。成功清除抗原后,PD-1表達(dá)下降。此外,Pdcd1 mRNA在前B細(xì)胞階段在發(fā)育中的B淋巴細(xì)胞中表達(dá)。PD-1的結(jié)構(gòu)包括一個ITIM(免疫受體酪氨酸抑制基序),表明PD-1負(fù)調(diào)節(jié)TCR信號。PD-1通過結(jié)合它的兩個配體PD-L1和PD-L2發(fā)出信號,這兩個配體都是B7家族的成員。配體結(jié)合后,PD-1信號抑制T細(xì)胞活化,導(dǎo)致增殖、細(xì)胞因子產(chǎn)生和T細(xì)胞死亡減少。此外,已知PD-1在小鼠的外周耐受性和預(yù)防自身免疫性疾病中起關(guān)鍵作用,因?yàn)镻D-1敲除動物表現(xiàn)出擴(kuò)張性心肌病、脾腫大和外周耐受性喪失。誘導(dǎo)的PD-L1表達(dá)常見于許多腫瘤,包括鱗狀細(xì)胞癌、結(jié)腸腺癌和乳腺腺癌。PD-L1過度表達(dá)導(dǎo)致腫瘤細(xì)胞對CD8 T細(xì)胞介導(dǎo)的裂解的抗性增加。在黑色素瘤的小鼠模型中,通過用阻斷PD-L1和它的受體PD-1之間的相互作用的抗體治療,腫瘤生長可以暫時被抑制。目前PD-1是免疫療法作為癌癥治療的熱門靶點(diǎn)之一。

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產(chǎn)品詳情:

產(chǎn)品名稱

RecombiMAb anti-mouse PD-1 (CD279) (D265A)

產(chǎn)品貨號

CP002

產(chǎn)品規(guī)格

1mg

反應(yīng)種屬

Mouse

克隆號

RMP1-14-CP002

同種型

Mouse IgG1(switched from rat IgG2a)

免疫原

Syrian Hamster BKH cells transfected with mouse PD-1 cDNA

實(shí)驗(yàn)應(yīng)用

in vivo blocking of PD-1/PD-L signaling*

*Reported for the original rat IgG2a RMP1-14 antibody

產(chǎn)品形式

PBS, pH 7.0,Contains no stabilizers or preservatives

純度

>95%, Determined by SDS-PAGE

聚合

<5%, Determined by SEC

無菌處理

0.2 μm filtration

純化方式

Protein G

分子量

150 kDa

小鼠病原檢測

Ectromelia/Mousepox Virus: Negative

Hantavirus: Negative

K Virus: Negative

Lactate Dehydrogenase-Elevating Virus: Negative

Lymphocytic Choriomeningitis virus: Negative

Mouse Adenovirus: Negative

Mouse Cytomegalovirus: Negative

Mouse Hepatitis Virus: Negative

Mouse Minute Virus: Negative

Mouse Norovirus: Negative

Mouse Parvovirus: Negative

Mouse Rotavirus: Negative

Mycoplasma Pulmonis: Negative

Pneumonia Virus of Mice: Negative

Polyoma Virus: Negative

Reovirus Screen: Negative

Sendai Virus: Negative

Theiler’s Murine Encephalomyelitis: Negative

保存條件

抗體原液保存在4°C,不能冷凍保存。

推薦抗體稀釋液

InVivoPure pH 7.0 Dilution Buffer(貨號IP0070)

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該產(chǎn)品自上市已被多篇SCI文獻(xiàn)引用,品質(zhì)有保證,以下是部分已發(fā)表的文獻(xiàn)引用:

應(yīng)用

文章

體內(nèi)PD-1/PD-L信號阻斷

(in vivo blocking of PD-1/PD-L?

signaling)

1.?Triplett, T. A., et al. (2018). 'Reversal of indoleamine 2,3-dioxygenase-mediated cancer?

immune suppression by systemic kynurenine depletion with a therapeutic enzyme' Nat?

Biotechnol 36(8): 758-764.


2.?Grasselly, C., et al. (2018). 'The Antitumor Activity of Combinations of Cytotoxic?

Chemotherapy and Immune Checkpoint Inhibitors Is Model-Dependent' Front Immunol 9: 2100.

3.?Moynihan, K. D., et al. (2016). 'Eradication of large established tumors in mice by?

combination immunotherapy that engages innate and adaptive immune responses' Nat?

Med. doi : 10.1038/nm.4200.


4.?Ngiow, S. F., et al. (2015). 'A Threshold Level of Intratumor CD8+ T-cell PD1 Expression?

Dictates Therapeutic Response to Anti-PD1' Cancer Res 75(18): 3800-3811. ?


5.?Evans, E. E., et al. (2015). 'Antibody Blockade of Semaphorin 4D Promotes Immune?

Infiltration into Tumor and Enhances Response to Other Immunomodulatory Therapies'?

Cancer Immunol Res 3(6): 689-701.


6.?Zelenay, S., et al. (2015). 'Cyclooxygenase-Dependent Tumor Growth through Evasion?

of Immunity' Cell 162(6): 1257-1270.


7.?Zander, R. A., et al. (2015). 'PD-1 Co-inhibitory and OX40 Co-stimulatory Crosstalk?

Regulates Helper T Cell Differentiation and Anti-Plasmodium Humoral Immunity' Cell?

Host Microbe 17(5): 628-641.

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