BioXCell熱銷產(chǎn)品--RecombiMAb anti-mouse CSF1R (CD115)
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產(chǎn)品描述：
AFS98-CP131單克隆抗體是原始AFS98單克隆抗體的重組嵌合型抗體。可變結(jié)構(gòu)域序列與原始AFS98單克隆抗體相同，但是恒定區(qū)序列已經(jīng)從大鼠IgG2a變到小鼠IgG2a。AFS98-CP131單克隆抗體像原始大鼠IgG2a抗體一樣，不包含F(xiàn)c突變。
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AFS98-CP131單克隆抗體能與小鼠集落刺激因子1受體(CSF1R)，也稱為巨噬細胞集落刺激因子受體(M-CSFR)，即CD115反應。CSF1R是一種單程I型膜蛋白，是血小板衍生生長因子受體家族的成員。在小鼠中，CSF1R由單核細胞/巨噬細胞、腹膜滲出物細胞、漿細胞樣和常規(guī)樹突細胞以及破骨細胞表達。CSF1R是CSF1的受體，CSF1通過CSF1R調(diào)節(jié)單核細胞譜系中細胞的增殖和分化。據(jù)報道，AFS98單克隆抗體可在體內(nèi)deplete巨噬細胞并阻斷CSFR1。
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產(chǎn)品詳情：
產(chǎn)品名稱 | RecombiMAb anti-mouse CSF1R (CD115) |
產(chǎn)品貨號 | CP131 |
產(chǎn)品規(guī)格 | 1/5/25/50/100mg |
反應種屬 | Mouse |
克隆號 | AFS98-CP131 |
同種型 | Mouse IgG2a(switched from rat IgG2a) |
免疫原 | Not available or unknown |
實驗應用 | in vivo?macrophage depletion*
in vitro?CSF1R neutralization*
in vivo?monocyte depletion*
Flow cytometry*
Western blot*
*Reported for the original rat IgG2a AFS98 antibody |
產(chǎn)品形式 | PBS, pH 7.0,Contains no stabilizers or preservatives |
純度 | >95%, Determined by SDS-PAGE |
聚合 | <5%, Determined by SEC |
無菌處理 | 0.2 μm filtration |
純化方式 | Protein A |
分子量 | 150 kDa |
小鼠病原檢測 | Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theiler’s Murine Encephalomyelitis: Negative |
保存條件 | 抗體原液保存在4°C，不能冷凍保存。 |
推薦同型對照 | InVivoPlus mouse IgG2a isotype control, unknown specificity（貨號BP0085） |
推薦抗體稀釋液 | InVivoPure pH 7.0 Dilution Buffer（貨號IP0070） |
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該產(chǎn)品自上市已被多篇SCI文獻引用，品質(zhì)有保證，以下是部分已發(fā)表的文獻引用：
| 應用 | 文章 |
體內(nèi)巨噬細胞耗竭 (in vivo macrophage? depletion) | 1.?Bauche, D., et al. (2018). 'LAG3(+) Regulatory T Cells Restrain Interleukin-23-Producing? CX3CR1(+) Gut-Resident Macrophages during Group 3 Innate Lymphoid Cell-Driven Colitis'? Immunity 49(2): 342-352 e345. 2.?Gordon, S. R., et al. (2017). 'PD-1 expression by tumour-associated macrophages inhibits? phagocytosis and tumour immunity' Nature 545(7655): 495-499. 3.?Moynihan, K. D., et al. (2016). 'Eradication of large established tumors in mice by combination? immunotherapy that engages innate and adaptive immune responses' Nat Med. doi : 10.1038/nm.4200. 4.?Naik, S., et al. (2015). 'Commensal-dendritic-cell interaction specifies a unique protective? skin immune signature' Nature 520(7545): 104-108. |
體外CSF-R1中和 (in vitro CSF-R1? neutralization) | 1.Sheng, K. C., et al. (2014). 'IL-3 and CSF-1 interact to promote generation of? CD11c+IL-10-producing macrophages' PLoS One 9(4): e95208.? |
流式細胞術(shù) (Flow Cytometry) | 1.?Li, W., et al. (2012). 'Intravital 2-photon imaging of leukocyte trafficking in beating? heart' J Clin Invest 122(7): 2499-2508.? 2.?Tagliani, E., et al. (2011). 'Coordinate regulation of tissue macrophage and dendritic? cell population dynamics by CSF-1' J Exp Med 208(9): 1901-1916. |
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