轉(zhuǎn)錄因子報(bào)告基因細(xì)胞（人源和鼠源細(xì)胞系）

2023-02-22

瀏覽次數(shù)： 82

InvivoGen提供一系列人源和鼠源細(xì)胞系以供研究由PRR配體或細(xì)胞因子激發(fā)的信號(hào)級(jí)聯(lián)反應(yīng)中蛋白質(zhì)發(fā)揮的作用。

在具有NF-κB, IRFs, NFAT, 或AhR轉(zhuǎn)錄因子元件的啟動(dòng)子的控制下，這些細(xì)胞均可以表達(dá)SEAP分泌型堿性磷酸酶或Lucia熒光素酶報(bào)告基因。

部分細(xì)胞系會(huì)敲除特定表達(dá)基因以達(dá)到篩選的目的。

所有細(xì)胞系均通過不同方法進(jìn)行嚴(yán)格的生物活性檢測(cè)（PCR, DNA測(cè)序，Western- Blot, FACS以及功能驗(yàn)證）。

轉(zhuǎn)錄因子報(bào)告基因細(xì)胞（人源）

細(xì)胞系	產(chǎn)品名稱	通路概述	報(bào)告系統(tǒng)*	貨號(hào)
HUMAN REPORTER CELLS
A549	A549-Dual?	NF-κB & IRFs	SEAP / Lucia	a549d-nfis
HCT116	HCT116-Dual?	NF-κB & IRFs	SEAP / Lucia	hctd-nfis
HEK293	HEK-Blue? ISG	IRFs	SEAP	hkb-isg
	293-Dual? Null	Double readout** (IRFs/ IFN-β), Lucia knocked into IFN-β	SEAP / Lucia	293d-null
	HEK-Dual? Null	Double readout** (NF-κB / IL-8), Lucia knocked into IL-8	SEAP / Lucia	hkd-nullni
HepG2	HepG2-Dual?	NF-κB & IRFs	SEAP / Lucia	hepg2d-nfis
HepG2	HepG2-Lucia? AhR	AhR	Lucia	hpgl-ahr
HT29	HT29-Lucia? AhR	AhR	Lucia	ht2l-ahr
Jurkat	Jurkat-Dual?	NF-κB & IRFs	Lucia / SEAP	jktd-isnf
	Jurkat-Lucia? NFAT	NFAT	Lucia	jktl-nfat
	Jurkat-Lucia? NFAT-CD16	NFAT, human CD16A (FcγRIIIA, V158 allotype) transfected	Lucia	jktl-nfat-cd16
Ramos	Ramos-Blue?	NF-κB / AP-1	SEAP	rms-sp
Ramos	Ramos-Blue? KD-MyD	NF-κB / AP-1, knockdown of human MyD88	SEAP	rms-kdmyd
THP-1	THP1-Blue? NF-κB	NF-κB	SEAP	thp-nfkb
	THP1-Lucia? NF-κB	NF-κB	Lucia	thpl-nfkb
	THP1-Blue?-ISG	IRFs	SEAP	thp-isg
	THP1-Lucia? ISG	IRFs	Lucia	thpl-isg
	THP1-Dual?	NF-κB & IRFs	SEAP / Lucia	thpd-nfis
	THP1-Dual? KO-IRF1	NF-κB & IRFs, knockout of human IRF1	SEAP / Lucia	thpd-koirf1
	THP1-Dual? KO IRF3	NF-κB & IRFs, knockout of human IRF3	SEAP / Lucia	thpd-koirf3
	THP1-Dual? KO IRF5	NF-κB & IRFs, knockout of human IRF5	SEAP / Lucia	thpd-koirf5
	THP1-Dual? KO IRF7	NF-κB & IRFs, knockout of human IRF7	SEAP / Lucia	thpd-koirf7

轉(zhuǎn)錄因子報(bào)告基因細(xì)胞（鼠源）

轉(zhuǎn)錄因子報(bào)告基因細(xì)胞

關(guān)鍵信號(hào)基因敲除細(xì)胞（人源和鼠源）

細(xì)胞系	產(chǎn)品名稱	通路概述	報(bào)告系統(tǒng)*	貨號(hào)
HUMAN REPORTER CELLS
THP1	THP1-Dual? KO-IFNAR2	NF-κB & IRFs, knockout of human IFNAR2	SEAP / Lucia	thpd-koifnar2
	THP1-Dual? KO-IKK?	NF-κB & IRFs, knockout of human IKK?	SEAP / Lucia	thpd-koikke
	THP1-Dual? KO-MyD	NF-κB & IRFs, knockout of human MyD88	SEAP / Lucia	thpd-komyd
	THP1-Dual? KO-TBK1	NF-κB & IRFs, knockout of human TBK1	SEAP / Lucia	thpd-kotbk
	THP1-Dual? KO-TREX1	NF-κB & IRFs, knockout of human TREX1	SEAP / Lucia	thpd-kotrex
	THP1-Dual? KO-TRIF	NF-κB & IRFs, knockout of human TRIF	SEAP / Lucia	thpd-kotrif
MOUSE REPORTER CELLS
RAW	RAW-Lucia? ISG-KO-TBK1	IRFs, knockout of mouse TBK1	Lucia	rawl-kotbk
	RAW-Lucia? ISG-KO-TREX1	IRFs, knockout of mouse TREX1	Lucia	rawl-kotrex
	RAW-Lucia? ISG-KO-TRIF	IRFs, knockout of mouse TRIF	Lucia	rawl-kotrif